Saguaro

ChromaLive Non-Toxic Dye

Generate New Lead Compounds Faster

Dying HeLa cells stained with ChromaLive and captured on Yokogawa CQ1 Imager

A First-of-a-Kind Dye

ChromaLive

Unlock new dimensions for evaluating drug performance in
high-content screens

Unlocking Time and Scalability

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Non Toxic

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Unaffected gene expression enables live cell imaging over weeks

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Multiplex

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Quantify broad ranges of cell states, or hint at drug mode of action

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Automation Friendly

One-step, mix-and-read dye that does not fluoresce in media

Capture more with one dye

  • More timepoints
  • More phenotypes
  • More throughput

Get in touch

Reach out to learn more about ChromaLive

 

Applications

Mechanism of Action

Just like cell painting, MoA of new compounds can be predicted by comparing with compounds of known MoA. However, ChromaLive was found to improve robustness of MoA predictions, shorten turnaround time, and elucidate time-dependent phenotypic shifts.

Toxicity Testing

Identify toxic concentration thresholds of compounds with dose-response assays. Distinct cell death mechanisms triggered by compounds can be quantified by comparing their phenotypic profile to that of  compounds with known toxic effects.

Time-Lapse Assays​

Incorporate the dimension of time to bring new insight into the mechanisms of compound, genetic, or pathological perturbations.

ChromaLive Dye FAQ

RNA sequencing data and week-long cell cultures support this claim. Figure 2 of this SLAS Discovery article presents supporting RNA sequencing and proteomics data showing unperturbed expression patterns – in contrast with alternative live cell dyes – for cells cultured in presence of ChromaLive. 

In addition, ChromaLive has been successfully tested in sensitive cell systems without affecting cell health for weeks on end. For instance, it has been used in a 3-week culture of iPSC-derived neurons, and a 6-week culture of patient-derived prostate cancer organoids.

ChromaLive fluorescence intensity remains constant over long periods of time (i.e. weeks). The non-toxic nature of ChromaLive, along with its fluorescence only upon incorporation into cells, allows its constant presence in culture medium. As a result, any photo-bleached molecule would get replaced by other ChromaLive molecules present in the culture medium. 

For instance, a protocol of repeated light exposure (10 frames per second for 60 seconds) to iPSC-derived cardiomyocytes stained with ChromaLive has been successfully tested to produce a video of a beating network of cardiomyocytes without photobleaching.

ChromaLive is one product which is a multi-chromatic small molecule dye that is excited at two different wavelengths: 488nm, with a long-stokes shift emission in the red (making it compatible with your own GFP fluorophore) and 561nm which has standard yellow emission. See our document “How does ChromaLive work” in our resources for more details such as why it stains both cell cytoplasm and mitochoindria at the same time: https://www.saguarobio.com/resources/

Fluorescence signal from ChromaLive can both be retained or removed after fixation, depending on your downstream assay needs. Signal retention requires our ChromaLive Fix additive and is performed with 4% PFA. By fixing and permabilizing cells (without ChromaLive Fix additive) signal can be entirely removed.

Most high-content imaging instruments are compatible with ChromaLive. Refer to our resources on our website to find a list of compatible instruments, along with technical notes detailing filter selection specific to the Yokogawa, Molecular Devices and Revvity systems. Incucyte systems are also compatible with ChromaLive!

ChromaLive comes in tubes of 10uL, and is used at a dilution of 1:1,000 in culture medium. The biological inertness of ChromaLive and its fluorescence only upon incorporation into cells allow its addition at the cell seeding stage, which is highly recommended. Addition of ChromaLive at a later stage is possible, but it is recommended to test staining kinetics as optimal staining can take up to 12 hours depending on cell type.

Reach out for a quote! Volume pricing is available, and academic discounts too.

See our FAQ.

 

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