Discover new insights into Immune Cell Killing (ICK) assays with this application note that describes a novel application for ChromaLIVE™, a non-toxic dye used in live-cell imaging to provide continuous and quantitative monitoring of effector-target cell interactions within co-culture systems.

Conventional ICK assays often rely on endpoint measurements, which miss dynamic cellular interactions and subtle phenotypic changes over time. ChromaLIVE™ addresses these limitations by enabling the tracking of cellular dynamics with minimal biological interference, allowing for real-time cytotoxicity assessment and the identification of different phenotypic states over multiple days.

This application note demonstrates how ChromaLIVE™ is a valuable tool to gain deeper biological insights, for example by tracking time-dependent cancer cell death induced by activated T lymphocytes. It showcases the versatility of ChromaLIVE™  in supporting high-content, image-based phenotypic profiling to address key challenges in immunotherapy development and vaccine evaluation.