Drug Profiling Service
Our proprietary ChromaLIVE™ live-cell painting method is trusted by drug discovery leaders to generate enhanced drug annotations in image-based profiling.
Powered by the Yokogawa CQ1 High-Content Imager and advanced automation, to enable reproducible and high-quality data generation every time!
Our live cell assay specialists and bioinformatics wizards can help you with wet lab, imaging, and analysis.
Profile your compounds against a curated library of 3,000+ annotated molecules, including FDA-approved, JUMP cell painting , and COMAS reference compounds.
Start with U2OS cells, with liver, neuronal, and other models coming soon for broader biological annotations.
Gain insights with cluster analyses, classification, compound matching, bioactivity detection scores, or similar tools, powered by the most cutting-edge deep learning models
Image-based profiling goes beyond traditional screening and omics profiling methods, by capturing the complexity of cellular responses in their native environment. These multidimensional datasets allow researchers to classify drugs more accurately, and uncover mechanisms of action that might otherwise remain hidden.
Our team of experts will help you unlock a more comprehensive view of drug behavior - bridging the gap between cell biology and actionable drug insights.
ChromaLIVE™ is a non-toxic, multi-chromatic dye that provides high-density biological information, and therefore helps differentiate subtle phenotypic signatures. ChromaLIVE™ is the leading technology for image-based profiling, providing the best recall and precision, ultimately translating to hit enrichment with minimal false negatives and false positives. Learn more here.
RNA sequencing data and week-long cell cultures support this claim. Figure 2 of this SLAS Discovery article presents supporting RNA sequencing and proteomics data showing unperturbed expression patterns – in contrast with alternative live cell dyes – for cells cultured in presence of ChromaLIVE.
In addition, ChromaLIVE™ has been successfully tested in sensitive cell systems without affecting cell health for weeks on end. For instance, it has been used in a 3-week culture of iPSC-derived neurons, and a 6-week culture of patient-derived prostate cancer organoids.
ChromaLIVE™ is a wash-free, multi-chromatic small molecule dye that is excited at two different wavelengths: 488nm, with a long-stokes shift emission in the red (making it compatible with your own GFP fluorophore) and a 561nm excitation (or 640nm excitation for our ChromaLIVE™ Deep Red).
See our document “How does ChromaLIVE™ work” in our resources for more details such as how it stains multiple cell compartments at the same time: https://www.saguarobio.com/resources/
ChromaLIVE™ is multichromatic and is therefore excited at two wavelengths: 488nm and 561nm. The 488 channel has a long-stokes shift emission in the red (making it compatible with your own GFP fluorophore), while the 561 channel has standard yellow emission. Most high-content imaging instruments are compatible with ChromaLIVE™. Refer to our resources on our website to find a list of compatible instruments, along with technical notes detailing filter selection specific to the Yokogawa, Molecular Devices and Revvity systems. Incucyte systems are also compatible with ChromaLIVE™!
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