This poster, in collaboration with NTNU and Mayo Clinic, reveals how NucleoLIVE™, our non-toxic nuclear dye, revolutionizes long-term live imaging of 2D and 3D cell cultures.

NucleoLIVE™ effectively overcomes the limitations of existing nuclear dyes that exhibit toxicity in long-term assays, compromising their effectiveness for live-cell imaging and real-time health assessments. As a mix-and-read non-toxic nuclear probe, it enables real-time long-term live-cell imaging of 2D and 3D cell cultures.

The poster presents NucleoLIVE™'s primary validation of non-toxicity, demonstrating no impact on cell proliferation over 6 days. Its compatibility for multiplexing with other fluorophores offers deeper insights into compound mechanisms through precise cell counts and real-time quantification. See how NucleoLIVE™ proves superior for drug screening in 3D spheroids and organoids, demonstrating excellent penetration and uniform staining. Its robust performance enables real-time visualization and quantification of nuclear dynamics, facilitating precise tracking of cell proliferation and responses to stimuli over time, even in complex 3D models.