Live-dead assays provide a quick and quantifiable way to measure the effect on cell viability and cytotoxicity of an experimental drug.
Because they are ironically toxic to cells, live-dead assays only capture a snapshot of cell viability and miss critical kinetic information. Saguaro's MortaLIVE non-toxic dye makes it possible to track cell viability and cytotoxicity in real-time, and allow scientist to more accurately identify promising drug candidates, while eliminating drugs that cause unwanted harm to cells.
Real-time and non-perturbing assessment of cell viability and cytotoxicity
Non-Toxic
Non-perturbing to cells, allowing real-time quantification of cell health over multiple days
Mix-and-read
One-step, no-wash dye which remains in media throughout the assay.
Fast and sensitive
Use both green and red fluorescence to quickly and sensitively discriminate live from dead cells
Saguaro's MortaLIVE uses the following two fluorescent channels (exc/em): 500nm / 520nm (dead), and 665nm / 680nm (live).
Cells that have their cellular membrane compromised are stained in green, and are therefore considered dead, whereas living cells will be stained in red and loose their red fluorescence when losing their metabolic activity. Loss of red fluorescence does not mean that cells are dead.
Join the 150+ biotech and pharma companies using Saguaro's non-toxic dyes for new insights
