Here's what ChromaLIVE can be used for in Live Cell Painting applications: This powerful tool reveals time-dependent phenotypic changes in compound-treated cells. Developed as a multi-chromatic, non-toxic dye designed for live-cell imaging, ChromaLIVE addresses the limitations of typical fixed endpoint assays in high-content screening that often miss crucial kinetic information, providing high-density biological information without disrupting cellular physiology.

We utilized ChromaLIVE to collect 7 kinetic measurements of MCF-7 cells, analyzing features such as intensity, texture, and shape. This approach precisely discriminates fast- from slow-acting compounds, identifies transient phenotypes and distinguishes compounds with similar mechanisms of action.

This application note highlights the benefits of kinetic phenotypic profiling with ChromaLIVE for gaining deeper insight into complex pharmacodynamics, validating it as a versatile and insightful tool for high-resolution exploration of cell phenotypes in drug discovery.