See this comprehensive tutorial on using CellProfiler Analyst's supervised classifier for advanced phenotypic profiling in live cells, specifically leveraging ChromaLIVE data. This guide addresses how to process data for high-content imaging and high-content screening in live cells experiments.

The tutorial details both unsupervised (dimensionality reduction, clustering with StratoMineR™) and supervised (classifier training with CellProfiler Analyst's Classifier module) data analysis approaches. It demonstrates how ChromaLIVE, a versatile non-toxic dye, enables the detection of a multitude of cellular phenotypes and the quantification of time-sensitive cellular events, making it ideal for Real-time cell viability assay and cytotoxicity assay applications.

Key applications include predicting drug mechanisms of action, assessing toxic doses, and understanding complex pharmacodynamics through kinetic assays. The guide highlights ChromaLIVE's unprecedented biocompatibility, mix-and-read protocol and suitability for extended incubation, even in 3D cell models for 3D cell imaging. By providing methods for precise image-based profiling in live cells, this tutorial equips researchers to uncover subtle phenotypes and accelerate drug discovery efforts.