Explore this standard protocol for 3D cell cultures using ChromaLIVE non-toxic dye. This detailed guide outlines the steps needed to leverage ChromaLIVE for time-dependent phenotypic changes in 3D cell models. As a multi-chromatic live-cell imaging dye, it offers a straightforward, mix-and-read protocol that eliminates washing steps.

The protocol covers comprehensive stages from dye preparation to detailed cell culture methodology for 3D cell cultures. It includes instructions for preparing Matrigel-coated plates, seeding cells in ChromaLIVE/Matrigel medium and optional Hoechst labeling for nuclear segmentation. For compatibility with our NucleoLIVE non-toxic nuclear dye, please refer to our ChromaLIVE Deep Red product.  

Further sections detail compound preparation and testing, and optimal imaging and data acquisition at desired timepoints. This protocol highlights ChromaLIVE's utility in 3D cell cultures for observing cellular responses, such as ER stress induced by Thapsigargin, across various timepoints and concentrations.