Explore this technical note introducing ChromaLIVE™ Deep Red, a novel non-toxic dye optimized for compatibility with NucleoLIVE™ Red. This new variation of our signature ChromaLIVE™ stain addresses limitations in traditional endpoint high-content screening and cell painting assays that overlook valuable kinetic information and transient cellular phenotypes.

The combination of ChromaLIVE™ Deep Red and NucleoLIVE™ Red offers unparalleled potential for precise nuclear segmentation and detailed morphological analysis across multiple cellular compartments, all while preserving the integrity of live cells. It retains the mix-and-read and multi-compartmental staining properties of its predecessor, with ChromaLIVE561 now observed in the 640 channel, freeing up the 561 channel for NucleoLIVE™ Red co-staining.

This non-toxic and live-cell compatible solution enables real-time, high-content phenotypic profiling, ideal for longitudinal kinetic assays and real-time drug screening applications. Its no-wash, mix-and-read protocol simplifies workflows and allows for multiplexing with NucleoLIVE™ Red to replace potentially toxic DNA dyes like Hoechst for safer, long-term imaging.