Explore this protocol for ChromaLIVE™ Fix Additive, a solution designed to retain ChromaLIVE fluorescence signal after cell fixation, overcoming the challenge of signal loss in traditional fixed-cell methods. This additive is part of our non-toxic dye family, ensuring the complete retention of ChromaLIVE signal, preserving its utility for downstream analysis and making it compatible with various experimental needs, including downstream antibody staining when the signal is removed through permeabilization.

The document outlines two convenient modes of use for the Additive:

 Mode 1, ideal for labs equipped with a microplate washer, involves replacing half the existing volume with a 2x Additive preparation, ensuring minimal disturbance.

Mode 2, recommended for labs without a microplate washer, simplifies the assay by adding 1/5 of the existing volume with a 6x Additive preparation.

Both modes facilitate a clear and quantifiable signal, enhancing the versatility of ChromaLIVE in fixed-cell applications.