Live cell painting vs fixed cell painting: phenotypic profiling with ChromaLIVE™ non-toxic dye and traditional cell painting

Live cell painting vs fixed cell painting:  phenotypic profiling with ChromaLIVE™ non-toxic dye and traditional cell painting

Discover how ChromaLIVE™ redefines Live Cell Painting with unsurpassed performance for phenotypic profiling. This technical note showcases ChromaLIVE™ as a non-toxic dye that excels in live-cell imaging, boosting bioactive compound detection and minimizing false predictions compared to traditional Cell Painting. 

ChromaLIVE™ streamlines phenotypic screens through its mix-and-read protocol, requiring no wash or fixation steps, allowing for capturing evolving phenotypes over an indefinite number of timepoints. Its non-toxicity ensures cells remain unperturbed, providing a more complete view of cell response. As a data-rich dye with dual excitation, ChromaLIVE™ is a powerful tool for image-based profiling in live cells. It is ideal for live-cell imaging in 3D cultures like patient-derived organoids.

This data highlights ChromaLIVE™'s superior ability to reveal subtle phenotypes and provide more accurate predictions for various mechanisms of action, making it a key solution for advanced biological insights.