Explore live cell painting, a novel approach for enhanced drug discovery by exploring time-dependent phenotypic changes. This poster is from a collaborative effort between Saguaro Biosciences, the Institute for Research in Immunology and Cancer and Sunnybrook Research Institute.

The method that is described leverages ChromaLIVE, our data-rich and non-toxic dye, offering performance similar to fixed cell painting but on live cells via live-cell imaging, thereby providing new mechanistic insights. It offers advantages such as revealing information on cellular responses across time and obviating washing and fixation, which prevents cell damage and loss.

It is also used for discriminating fast-acting from slow-acting compounds, identifying transient phenotypes and understanding similar mechanisms of action. Utilizing 2D cell cultures with kinetic measurements, live cell painting with ChromaLIVE streamlines workflows and can boost detection rates of bioactive compounds during screening campaigns. It enables robust high-content screening in live cells and powerful image-based profiling in live cells, proving to be a versatile tool for high-resolution exploration of cell phenotypes.