Explore cellular phenotypic profiling by combining live-cell imaging and cell painting techniques, leveraging ChromaLIVE non-toxic dye. This innovative approach applies cell painting technology in live cells to measure dynamic phenotypic changes, overcoming the need for cell fixation. ChromaLIVE enables the detection of various cellular states like apoptosis and ER stress.

Utilizing a live-cell kinetic mode and high-content imaging (Opera Phenix Plus), this method extracts thousands of features for robust image-based profiling in live cells. The study from Institut Pasteur Korea demonstrates the utility of live cell dyes for compound profiling in an effortless workflow, helping predict drug's mechanism of action.