Cell viability and cell death assays are essential tools to evaluate compound cytotoxicity in drug discovery. While many commercial solutions exist, most are endpoint assays that only capture a snapshot at a single timepoint.

MortaLIVE™ is a non-toxic, multichromatic dye developed specifically for live/dead assays and real-time live-cell imaging. Added directly to cell culture medium, it requires no fixation or washing and can be imaged repeatedly on standard platforms over several days.

By quantifying fluorescence in two distinct channels (“LIVE”/“Mortality”), researchers can monitor cell viability and death with precision. This dual-channel readout reveals how compounds affect cells progressively, impairing viability at lower doses before triggering cell death at higher concentrations.

MortaLIVE™ enables robust, image-based profiling in both 2D and 3D cultures, supporting high-content imaging workflows, real-time cytotoxicity assays, and live-cell painting strategies, all through a simple, mix-and-read protocol.