Optimize your live-cell assay and cytotoxicity assay assessment with MortaLIVE™, one of Saguaro Biosciences' non-toxic dyes. MortaLIVE™ is a non-toxic cell viability dye that enables real-time monitoring of cytotoxicity, crucial for high-content imaging and real-time cell viability assay applications. Unlike traditional toxic live-dead stains that only capture a snapshot of cell viability, Saguaro's non-toxic dye MortaLIVE™ provides continuous, real-time tracking for a better understanding of toxic liabilities and drug mechanisms.

It is non-perturbing to cells, allowing real-time quantification of cell health over multiple days. Added directly to cell culture media, this one-step, no-wash dye simplifies quantification of both live and dead cells across multiple timepoints by remaining in the media throughout the assay. As a mix-and-read, fast and sensitive fluorescent assay, it uses both green and red fluorescence to quickly and sensitively discriminate live from dead cells. By measuring fluorescence in the "LIVE" (excitation at 640nm) and "Mortality" (excitation at 488nm) channels, researchers can assess how compound concentrations first impair viability, then induce cell death. Ideal for imaging of 3D cell cultures and live-cell imaging in 3D culture.

Learn its simple protocol, including dye dilution and preparation , recommended incubation times and optimal storage conditions.