Discover the power of Live Cell Painting with ChromaLIVE™!

This poster explores time-dependent phenotypic changes for enhanced drug discovery. ChromaLIVE, a novel non-toxic dye with high-density biological information, enables live cell phenotypic profiling, offering new mechanistic insights. The poster explores how Live Cell Painting complements fixed endpoint assays by quantifying intermediate/transient phenotypes and discriminating compounds with similar MOA.

Through extensive kinetic measurements and time-resolved UMAPs, the study reveals new insights, allowing for differentiation of fast- from slow-acting compounds, transient from endpoint phenotypes, and even subtle mechanistic differences between colocalizing compounds. Live Cell Painting with ChromaLIVE emerges as a versatile tool for high-resolution exploration of cell phenotypes in drug discovery, boosting detection rates of bioactive compounds and providing a deeper understanding of compound mechanisms of action.