Explore this poster presented at SLAS 2024, a significant collaborative effort involving Saguaro Technologies, Sunnybrook Research Institute, IRIC - Université de Montreal, Plexium and contributions from the Broad Institute of MIT and Harvard. This work focuses on Live Cell Painting for probing in vitro compound toxicity in high-content screens with a novel non-toxic, mix-and-read method.

This poster highlights how ChromaLIVE, our novel non-toxic dye, offers the advantage of conducting image-based profiling on live cells, providing new mechanistic insight and overcoming challenges associated with fixed cells.

Applications include :

- predicting drug mode of action

- assessing toxic concentration thresholds

- evaluating toxic effects indefinitely over time.

The study validates ChromaLIVE's unprecedented biocompatibility at the genome, proteome, and cell level. Its potential use in Live Cell Imaging assays is confirmed, allowing quantification of time-sensitive cellular events like apoptosis and autophagy in live kinetic imaging.

A comparison with standard Cell Painting assays on 90 annotated compounds showed ChromaLive yielding the highest mean average precision (mAP) and comparable or better MoA prediction performance. Furthermore, ChromaLive has been successfully used for toxicity assessment in 3D cell cultures, leading to the identification of hit compounds on patient-derived organoids. This mix-and-read feature contributes to its high replicability scores, making it an ideal choice for predicting compound safety profiles from large-scale libraries.