This poster presents a novel approach for quantifying apoptosis in live cells. It introduces ChromaLIVE, our non-toxic dye designed for live-cell imaging and automated AI analysis, which addresses the limitations of traditional fixed-cell methods that compromise cell viability and miss dynamic apoptotic events.

The approach combines ChromaLIVE with ViQi's AutoHCS™ tool for high-content imaging analysis, allowing real-time visualization and quantification of apoptosis without harming cells. ChromaLIVE excels at quantifying time-sensitive cellular events, enhancing drug efficacy assessment, and providing a more nuanced understanding of apoptosis.

Applications include:

• predicting drug mode of action

• assessing toxic concentration thresholds

• evaluating toxic effects indefinitely over time.

The study validates ChromaLIVE's unprecedented biocompatibility at the genome, proteome, and cell level. Its potential in Live Cell Imaging assays for Real-time cell viability assay and cytotoxicity assay allows quantification of time-sensitive cellular events. As ChromaLIVE is ideal for high-content screening in live cells, it offers researchers a seamless, powerful, and unbiased way to circumvent traditional limitations and gain insights into complex disease mechanisms.