Technical note – Imaging ChromaLIVE™ non-toxic dye on the ImageXpress Micro Confocal (IXM Confocal)

Technical note – Imaging ChromaLIVE™ non-toxic dye on the ImageXpress Micro Confocal (IXM Confocal)

 

This technical note provides step-by-step instructions for imaging ChromaLIVE, a multi-chromatic live-cell imaging dye, on the ImageXpress Micro Confocal (IXM Confocal) high-content imaging systems. It also details how to create custom settings for optimal imaging of live samples.

ChromaLIVE reveals distinct cellular staining patterns when excited around 488nm or 561nm. The document explains how specific filters on the IXM instrument allow selective imaging of each staining pattern. It provides detailed instructions for setting up filters. This ensures users can fully benefit from ChromaLIVE's technology by imaging cells at multiple wavelengths on IXM Confocal and IXM Confocal HT.ai systems.

 

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