This work highlights a novel fluorescent non-toxic dye (ChromaLIVE), enabling real-time phenotypic profiling in live cells and the quantification of disease-relevant phenotypes like apoptosis, autophagy, and ER stress. ChromaLIVE's unique properties, such as its multi-chromatic...

  Explore this standard protocol for 3D cell cultures using ChromaLIVE non-toxic dye. This detailed guide outlines the steps needed to leverage ChromaLIVE for time-dependent phenotypic changes in 3D cell models. As a multi-chromatic live-cell...

  Explore Live Cell Painting with ChromaLIVE, a novel non-toxic method enhancing throughput and insights in drug discovery. This document highlights how our non-toxic dye enables image-based profiling in live cells, overcoming the limitations of...

  This tutorial outline a basic image analysis workflow for Live Cell Painting with ChromaLIVE™ in CellProfiler™. This guide details a simple workflow from preprocessing (uploading images, extracting metadata) to segmentation (identifying nuclei and cells)...

  See this comprehensive tutorial on using CellProfiler Analyst's supervised classifier for advanced phenotypic profiling in live cells, specifically leveraging ChromaLIVE data. This guide addresses how to process data for high-content imaging and high-content screening in...

  Explore this protocol for ChromaLIVE™ Fix Additive, a solution designed to retain ChromaLIVE fluorescence signal after cell fixation, overcoming the challenge of signal loss in traditional fixed-cell methods. This additive is part of our...

Explore cellular phenotypic profiling by combining live-cell imaging and cell painting techniques, leveraging ChromaLIVE non-toxic dye. This innovative approach applies cell painting technology in live cells to measure dynamic phenotypic changes, overcoming the need for...